ConviFlex™ DNAmp Mix is a lyophilized, ready-to-use PCR master mix, which consists of all core components required for PCR amplification, except DNA template and primers.
PCR amplification has become an indispensable method in every molecular biology lab. Usually, the necessary reaction components (e.g. buffer, dNTPs, MgCl2, and DNA polymerase) are sequentially added to prepare a master mix, which is then aliquoted to individual reaction tubes. The subsequent inclusion of reaction-specific primers and template DNA prior to PCR adds further pipetting steps to the procedure, thereby increasing the margin of error, the risk of cross-contaminations and work time.
Our ready-to-use and universal ConviFlex™ DNAmp Mix is pre-mixed, lyophilized, and optimized for efficient and reproducible PCR. The use of ConviFlex™ DNAmp Mix requires a minimum number of pipetting steps and hands-on time, reducing the risk of cross-contaminations.
ConviFlex™ DNAmp Mix is compatible with both conventional PCR and qPCR, and is suitable for all regular PCR applications including PCR assays with complex genomic or cDNA templates, low copy number targets, high specificity PCR, T/A cloning, and multiplex PCR. DNA template can be extracted from diverse starting materials like cell culture supernatants, bacterial colonies, and mammalian cells and tissues.
ConviFlex™ DNAmp Mix is also suitable for PCR amplification using DNA template directly from cell culture supernatants or bacterial suspensions.
ConviFlex™ DNAmp Mix containing hot start Taq polymerase and dNTPs (100 reactions / vial)
MgCl2 (100 mM)
- Cat. No. 191-0100 100 reactions
- Cat. No. 191-0500 500 reactions
- Cat. No. 191-1000 1000 reactions
Microcentrifuge vials (1.5 ml), filter tips, PCR reaction tubes, user-specific primers and probes
Required Lab Devices
Microcentrifuge, Heat block, Pipettes, PCR thermocycler
Shelf Life and Storage
Lyophilized ConviFlex™ DNAmp Mix and Rehydration Buffer should be stored at +2 – +8 °C. After rehydration, ConviFlex™ DNAmp Mix should be stored at ≤ -18 °C and is stable even after several freeze/thaw cycles.