Description

PCR kit for conventional, endpoint PCR of bacteria in various in situ biologicals including cell cultures and virus stocks. The kit can detect more than 45 bacteria genera, based on alignment analysis.

Recommended Use

Applicable in research and industry for direct testing of cell cultures and biologicals. For QA application specific validation might be required. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components

Lyophilized Mix: Primers / Nucleotides / Internal Control DNA / Polymerase in aliquots of 25 reactions each.
Rehydration buffer
Lyophilized Positive Control DNA
PCR Grade Water

Package Sizes

• Cat. No. 12-1025 25 Reactions
  Cat. No. 12-1050 50 Reactions
  Cat. No. 12-1100 100 Reactions
  Cat. No. 12-1250 250 Reactions

Result Evaluation

Gel electrophoresis at endpoint of PCR

Required Consumables

PCR reaction tubes and filter tips

Required Lab Devices

PCR cycler
Agarose gel electrophoresis and DNA stain
Pipetting equipment
Microcentrifuge

Shelf Life and Storage

The kit should be stored at +2 to +8 °C for at least 6 months. After rehydration the reagents must be stored at ≤ -18 °C.

Fig. Amplified PCR products are visualized by standard gel electrophoresis. Amplification of the Internal Control DNA (low molecular weight band) indicates a successful PCR and the lack of PCR-inhibiting components in the sample. A band in correspondence of the target (ca. 460 bp) implies the presence of a bacterial contamination.