Description
PCR kit for conventional, endpoint PCR of bacteria in various in situ biologicals including cell cultures and virus stocks. The kit can detect more than 45 bacteria genera, based on alignment analysis.
Recommended Use
Applicable in research and industry for direct testing of cell cultures and biologicals. For QA application specific validation might be required. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.
Kit Components
Lyophilized Mix: Primers / Nucleotides / Internal Control DNA / Polymerase in aliquots of 25 reactions each.
Rehydration buffer
Lyophilized Positive Control DNA
PCR Grade Water
Package Sizes
- Cat. No. 12-1025 25 Reactions
- Cat. No. 12-105050 Reactions
- Cat. No. 12-1100100 Reactions
- Cat. No. 12-1250250 Reactions
Result Evaluation
Gel electrophoresis at endpoint of PCR
Required Consumables
PCR reaction tubes and filter tips
Required Lab Devices
PCR cycler
Agarose gel electrophoresis and DNA stain
Pipetting equipment
Microcentrifuge
Shelf Life and Storage
The kit should be stored at +2 to +8 °C for at least 6 months. After rehydration the reagents must be stored at ≤ -18 °C.
Fig. Amplified PCR products are visualized by standard gel electrophoresis. Amplification of the Internal Control DNA (low molecular weight band) indicates a successful PCR and the lack of PCR-inhibiting components in the sample. A band in correspondence of the target (ca. 460 bp) implies the presence of a bacterial contamination.
