SwabUp™ Lab Monitoring Plus kit with a contamination-free PCR system is for tracing DNA contamination hot-spots in molecular biology labs in order to efficiently eliminate them and prevent future occurrence.
Small amounts of amplicon- or target DNA contaminations could lead to PCR artifacts and false positive results. Originating from aerosolized fragments in centrifuges, pipettes and other lab equipment or from small splatters during working with open reaction tubes, contaminant DNA is very hard to remove and can lead to cross contaminations between samples and widespread problems throughout the testing procedure and interpretation of results. SwabUp™ Lab Monitoring Plus kit contains a sample collection system, a DNA extraction system, which was optimized for the efficient detection of the smallest amounts of contaminant DNA, and a contamination-free PCR System.
Recommended Use / Scope
This kit is recommended for the regular monitoring of the lab work area and detection of target or amplicon DNA contaminations, but especially for severe and persistent cases of DNA contamination, where every single reagent used for PCR could potentially be contaminated making it extremely difficult to trace the contamination source.
SwabUp™ Lab Monitoring Plus kit introduces the contamination-free SwabUp™ DNAmp Mix, a universal PCR master mix, which contains all PCR core components. Only reaction-specific primers and template DNA are added by the user.
SwabUp™ Lab Monitoring kit is for research use only. It is not recommended for clinical and diagnostic applications or for the detection of RNA contaminations.
Separately packed swabs, Collection Buffer tubes, spin column-based DNA extraction system, SwabUp™ DNAmp Mix, Rehydration Buffer, MgCl2.
- Cat. No. 182-0010 10 applications (25 PCR reactions)
- Cat. No. 182-0050 50 applications (125 PCR reactions)
Ethanol (> 96 % abs.), 1.5 ml reaction tubes, PCR tubes
Required Lab Devices
Microcentrifuge, heat block, pipetting equipment, PCR thermocycler
Shelf Life and Storage
DNA extraction system should be stored at room temperature, Collection Buffer tubes must be stored at 2 – 8 °C. Swabs can be stored at 2 – 30 °C. SwabUp™ DNAmp Mix should be stored at 2 – 8 °C, and after rehydration at < -18 °C.