PCR Quantification Standards

Our PCR Quantification Standards contain genomic DNA extracted from low passage of defined microorganisms. The DNA starting material was analyzed with standardized methods to determine the DNA’s integrity and quantity. Only pure and intact DNA showing a > 10 kb band in an agarose gel and an OD260/280 > 1.8 is used. The DNA was partially sequenced to confirm the species’ identity.

Scope of Application

PCR Quantification Standards contain a defined number of genome copies of a particular microorganism. Therefore, our standards are perfectly suitable for generating DNA standard curves in qPCR. In addition, standards are required for testing the specificity and sensitivity of PCR assays, particularly, in the context of quality control and in correspondence with EP 2.6.7. compliance

Kit Components

1 vial with 1×108 genomes (freeze-dried.)
3 vials with Tris buffer (10 mM, pH 8.5)

Available Species
  • Cat. No. 52-0116 Acholeplasma laidlawii
  • Cat. No. 52-5571 Bordetella pertussis
  • Cat. No. 52-0083 Escherichia coli
  • Cat. No. 52-0101 Legionella pneumophila
  • Cat. No. 52-0129 Mycoplasma arginini
  • Cat. No. 52-0117 Mycoplasma fermentans
  • Cat. No. 52-0115 Mycoplasma gallisepticum
  • Cat. No. 52-0130 Mycoplasma hyorhinis
  • Cat. No. 52-0112 Mycoplasma orale
  • Cat. No. 52-0119 Mycoplasma pneumoniae
  • Cat. No. 52-0103 Mycoplasma salivarium
  • Cat. No. 52-0124 Mycoplasma synoviae
  • Cat. No. 52-0164 Spiroplasma citri
  • Cat. No. 52-0071 Pseudomonas aeruginosa

The kit must be stored at 2-8 °C (Shipped at ambient temperatures).