Enzymes

Enzymes

Choosing the right enzymes for your PCR and molecular biology application can profoundly affect your experimental outcome.

The primary requirements for a DNA polymerase for PCR are optimal activity at temperatures around 75 °C and the ability to retain that activity after prolonged incubation at even higher temperatures (95 °C). The first thermostable DNA polymerase to be widely used for PCR was Taq DNA Polymerase.

For PCR applications that require optimization, a routine Taq DNA Polymerase might not be the right choice.

Minerva Biolabs´ hot-start Taq DNA Polymerase is a high-quality product with highly processive 5´→3´ polymerase and no 3´→5´ exonuclease activity. Conveniently inhibited by specific antibodies at room temperature (i.e. during the preparation of the samples), the MB Taq DNA Polymerase guarantees efficient and highly specific PCR reactions.

Activation of the Taq polymerase is initiated at temperatures above 70 °C and fully achieved at 94 °C after 2 minutes. At 72 °C and at ca. pH 9, MB Taq DNA Polymerase reaches its highest level of activity, capable of amplifying a standard 1 kb strand of DNA in approximately 30 seconds.

Find out more about our Taq Polymerase and our other enzymes for your
molecular biology and biochemical applications.

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