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Choosing the enzymes for PCR can profoundly affect the outcome of the PCR. As PCR became more sophisticated, PCR enzymes such as polymerase mixtures and blends began to be used.
The primary requirements for a DNA polymerase used in PCR are optimal activity at temperatures around 75°C and the ability to retain that activity after prolonged incubation at even higher temperatures (95°C).
The first thermostable DNA polymerase to be widely used for PCR was Taq DNA Polymerase. For many conventional PCRs that do not require extensive optimization, Taq DNA Polymerase is a good choice, but it is not the best you can do.
Minerva Biolabs have better options for you.
Recombinant Taq DNA Polymerase produces the best results keeping it a high-quality product Our PCR enzymes such as Taq DNA Polymerase is a highly processive 5´→3´ polymerase with no 3´→5´ exonuclease (proofreading) activity.
At 72 °C and around pH 9 conditions, MB Taq DNA Polymerase reaches its highest level of activity, capable of amplifying a standard 1 kb strand of DNA in approximately 30 seconds. The Taq‘s activity is blocked at ambient temperature, e.g. while preparing your sample. Reverse inhibition of the Taq polymerase is made possible when the temperature is above 70 °C. Complete activation of the polymerase is achieved at 94 °C for 2 minutes.
Discover more about our enzymes for PCR below.
DNA contamination in PCR is a serious problem in labs who use PCR-technology frequently.
Small amounts of amplicon- or target DNA contaminations could lead to PCR artifacts and unwanted false positive results.
Contaminant DNA originates from aerosolized fragments in centrifuges, pipettes, other lab equipment and from small splatters during working with open reaction tubes. It is very hard to remove and can lead to cross contaminations between samples forcing labs to experience widespread problems throughout the testing procedure and interpretation of results.
Minerva Biolabs has a solution for you.
Our lab monitoring products can be performed in regular time intervals on samples collected from surfaces and/or equipment which are easily exposed to target and amplicon DNA contaminations.
Our products are designed to help you monitor contamination in your lab.
Explore all of our lab monitoring products below.
PCR Cycler Validation
PCR amplification includes many steps involved in setting up the PCR reaction and carries more possibilities of contamination with every extra step.
The common reaction components (e.g. buffer, dNTPs, MgCl2, and DNA polymerase) are mixed together by lab operators to create a master PCR mix, which is then aliquoted to individual reaction tubes.
Reaction-specific primers and template DNA are added prior to PCR, adding further pipetting steps to the procedure. Every extra step added increases your margin of error.
Minerva Biolabs PCR Mixes will decrease the risks of cross-contamination and time spent making a quality PCR mix.
The application of ConviFlex™ DNAmp PCR Mix requires minimal hands-on time and reduces the risk of cross-contamination due to the minimal number of pipetting steps required to set up the PCR reaction.
Discover how to decrease your margin of error with Minerva’s specially designed PCR mixes below.
MB Taq Polymerase€30,80 – €108,10 Select options
Proteinase K€25,20 Add to cart
10CFU™ Mycoplasma Sensitivity Standards€261,30 – €1.406,60 Select options
PCR Quantification Standards€261,30 Select options
Genomic DNA Extracts€129,00 – €130,20 Select options
qPCR Cycler Check™€539,80 Add to cart
PCR Cycler Check™€214,90 – €429,80 Select options
SwabUp™ Lab Monitoring Plus€90,70 – €383,30 Select options
SwabUp™ Lab Monitoring€76,60 – €328,80 Select options
ConviFlex™ DNAmp Mix€126,00 – €794,50 Select options