Enzymes

Choosing the enzymes for PCR can profoundly affect the outcome of the PCR. As PCR became more sophisticated, PCR enzymes such as polymerase mixtures and blends began to be used.

The primary requirements for a DNA polymerase used in PCR are optimal activity at temperatures around 75°C and the ability to retain that activity after prolonged incubation at even higher temperatures (95°C).

The first thermostable DNA polymerase to be widely used for PCR was Taq DNA Polymerase.
For many conventional PCRs that do not require extensive optimization, Taq DNA Polymerase is a good choice, but it is not the best you can do.

Minerva Biolabs have better options for you.

Recombinant Taq DNA Polymerase produces the best results keeping it a high-quality product
Our PCR enzymes such as Taq DNA Polymerase is a highly processive 5´→3´ polymerase with no 3´→5´ exonuclease (proofreading) activity.

At 72 °C and around pH 9 conditions, MB Taq DNA Polymerase reaches its highest level of activity, capable of amplifying a standard 1 kb strand of DNA in approximately 30 seconds. The Taq‘s activity is blocked at ambient temperature, e.g. while preparing your sample. Reverse inhibition of the Taq polymerase is made possible when the temperature is above 70 °C. Complete activation of the polymerase is achieved at 94 °C for 2 minutes.

Discover more about our enzymes for PCR below.