Water Diagnostics - Legionella spp.

---

Aqua Screen^® PCR detection kits

Aqua Screen^® PCR Diagnostic Kits are used for DNA samples prepared by using the Aqua Screen^® Fast Extract procedure. All detection kits for qPCR are AFNOR XP T90-471 compliant. For qPCR two formats are available depending on the qPCR machine being used.

Aqua Screen^® PCR detection kits - detection of Legionella spp.

The supplied primer set is specific for a segment of the 16S rRNA region of the legionella genome. When using the Legionella spp. detection kit for conventional PCR, the amplified PCR product is 244 bp long and can be rendered visible directly in the agarose gel. The conventional PCR internal control amplicon is 150 bp in size. The qPCR variant is designed such that the specific target probe fluoresces at 520 nm. The qPCR internal control is detected by another probe emitting fluorescent light at 600 nm.

Ordering

for conventional PCR

Tests	Cat.-No.
25 reactions	33-1025
100 reactions	33-1100
250 reactions	33-1250

>>> Ordering Information

for qPCR Type 1

Tests	Cat.-No.
25 reactions	33-2025
100 reactions	33-2100
250 reactions	33-2250

>>> Ordering Information

for qPCR Type 2

Tests	Cat.-No.
25 reactions	33-4025
100 reactions	33-4100
250 reactions	33-4250

>>> Ordering Information

 

Instrument Compatibility

+ = recommended kit version
– = not compatible
o = untested but presumed to be compatible
V = validated

Kit Components

• primer sets and nucleotides at optimized concentrations
• internal amplification control
• positive control for easy result verification
• instruction manual