Water Diagnostics - Legionella spp.

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Aqua Screen^® PCR detection kits - detection of Legionella spp.

The supplied primer set is specific for a segment of the 16S rRNA region of the legionella genome. When using the Legionella spp. detection kit for conventional PCR, the amplified PCR product is 244 bp long and can be rendered visible directly in the agarose gel. The conventional PCR internal control amplicon is 150 bp in size. The real-time PCR variant is designed such that the specific target probe fluoresces at 520 nm. The real-time PCR internal control is detected by another probe emitting fluorescent light at 600 nm.

Aqua Screen^®Ls-qDual

AFNOR conform detection of Legionella spp. (see below)

Aqua Screen^®Ls

for conventional PCR

Including internal amplification and positive control



Tests	Cat.-No.
25 reactions	33-1025
100 reactions	33-1100
250 reactions	33-1250

for real-time PCR

ALs-QP with LightCycler^® and ABI Prism^® (7000, 7300, 7700, 7900), including Legionella spp.-specific probes, internal amplification and positive control.



Tests	Cat.-No.
25 reactions	33-2025
100 reactions	33-2100
250 reactions	33-2250

Kit Components

• primer sets and nucleotides at optimized concentrations
• positive and internal control for easy result verification
• instruction manual

Aqua Screen^®Ls-qDual

Water Diagnostics - Legionella spp.

ALs-qDual (FAM channel*) for the detection of Legionella spp. DNA via real-time PCR includes Legionella spp.-specific Scorpions and allows the external amplification of control DNA (“spike”) according to AFNOR XP T90-471.

Aqua Screen^®Ls-qDual

With all current real-time machines



Tests	Cat.-No.
25 reactions	33-5025
100 reactions	33-5100
250 reactions	33-5250

Note

We highly recommend the Aqua Screen^® Filtration Unit in combination with the Extraction Kit or Aqua Screen^® Fast Extract for an optimal preparation of the DNA extracts from your water samples.