Water Diagnostics - Legionella pneumophila

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Aqua Screen^® PCR detection kits

Aqua Screen^® PCR Diagnostic Kits are used for DNA samples prepared by using the Aqua Screen^® Fast Extract procedure. All detection kits for qPCR are AFNOR XP T90-471 compliant. For qPCR two formats are available depending on the qPCR machine being used.

Aqua Screen^® PCR detection kits - detection of Legionella pneumophila

The supplied primer set is specific for a segment of the mip region of the Legionella pneumophila genome. When using the Legionella pneumophila Detection Kit for conventional PCR, the amplified PCR product is 275 bp long and can be rendered visible directly in the agarose gel. The conventional PCR internal control amplicon is 191 bp in size. The qPCR variant is designed such that the specific target probe fluoresces at 520 nm. The qPCR internal control is detected by another probe emitting fluorescent light at 600 nm.

Instrument Compatibility

+ = recommended kit version
– = not compatible
o = untested but presumed to be compatible
V = validated

Kit Components

• primer sets and nucleotides at optimized concentrations
• internal amplification control
• positive control for easy result verification
• instruction manual