Water Diagnostics - Legionella pneumophila

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Aqua Screen^® PCR detection kits - detection of Legionella pneumophila

The supplied primer set is specific for a segment of the mip region of the Legionella pneumophila genome. When using the Legionella pneumophila Detection Kit for conventional PCR, the amplified PCR product is 275 bp long and can be rendered visible directly in the agarose gel. The conventional PCR internal control amplicon is 191 bp in size. The real-time PCR variant is designed such that the specific target probe fluoresces at 520 nm. The real-time PCR internal control is detected by another probe emitting fluorescent light at 600 nm.

Aqua Screen^®PCR Diagnostic Kits

for conventional PCR

• for agarose gel electrophoresis
• including internal amplification and positive control

for real-time PCR

• with LightCycler^® 1 & 2.0, ABI Prism^® (7000, 7300, 7700, 7900) and related devices, with FAM and ROX chanels

Kit Components

• primer sets and nucleotides at optimized concentrations
• positive and internal control for easy result verification
• instruction manual
• storage box

Aqua Screen^®Lp-qDual (AFNOR XP T90-471)

ALp-qDual (FAM channel*) for the detection of Legionella pneumophila DNA via real-time PCR includes Legionella pneumophila-specific Scorpions and allows the external amplification of control DNA (“spike”) according to AFNOR XP T90-471.

Aqua Screen^®Lp-qDual

• With all current real-time machines

Note

We highly recommend the Aqua Screen^® Filtration Unit in combination with the Extraction Kit or Aqua Screen^®Fast Extract for an optimal preparation of the DNA extracts from your water samples.