Food Control™ LFA
Food Control™ LFA is a PCR-kit for fast and reliable detection of foodborne pathogens via conventional PCR in combination with our reliable Lateral Flow Assay (LFA) evaluation technology.
Detecting bacterial contamination is a key aspect in food microbiology. Consequently, microbiological quality control programs are intensified throughout food chain production in order to minimize the risk of intoxicating consumers. Reliability, speed and robustness of a test system to detect the presence or absence, or even the degree of contamination with pathogens, becomes increasingly important. Food Control™ LFA is a diagnostic system for easy non-quantitative detection of presence or absence of foodborne pathogens in agricultural or food industry via conventional PCR and Lateral Flow Assay (LFA) evaluation technology.
Type of Assay
The assay is based on a one tube amplification with a conventional PCR cycler and a subsequent probe specific hybridization technique. The resulting interpretation is a simple readout of coloured bars on a lateral flow stripe.
Recommended Use / Scope
Can be used in research and industry for detection of pathogenic contamination in food or agricultural products. For research use only! Do not use for clinical diagnostic or testing of patient samples.
The kit is available for the detection of the following microorganisms via conventional PCR:
||invasion protein A (invA) gen|
|Yersinia enterocolitica||heat stable enterotoxin A gen|
|Shigella spp.||invasion plasmid antigen (ipaH6) gen|
O-acyltransferase (lpxA) gen
|Clostridium perfringens||phospholipase C alpha toxin (plc) gen|
Escherichia coli (EHEC)
|Shiga Toxin 1 (stx1) gen|
Escherichia coli (EHEC)
|Shiga Toxin 2 (stx2) gen|
|Escherichia coli O157||wbdR gen|
|Escherichia coli O104||wckD gen|
|Listeria spp.||invasion associated protein p60 (iap) gen|
|Listeria monocytogenes||listeriolysin O (hly) gen|
|Salmonella spp.||spacer-region between 16S and 23S RNA genes|
Package size 25 reactions each:
- Cat. No. 11-03-01-025 Salmonella enterica
- Cat. No. 11-03-02-025 Yersinia enterocolitica
- Cat. No. 11-03-03-025 Shigella spp.
- Cat. No. 11-03-04-025 Campylobacter spp.
- Cat. No. 11-03-05-025 Clostridium perfringens
- Cat. No. 11-03-06-025 Shiga Toxin 1
- Cat. No. 11-03-07-025 Shiga Toxin 2
- Cat. No. 11-03-08-025 Escherichia coli O157
- Cat. No. 11-03-09-025 Escherichia coli O104
- Cat. No. 11-03-10-025 Listeria spp.
- Cat. No. 11-03-11-025 Listeria monocytogenes
- Cat. No. 11-03-12-025 Salmonella spp.
Lateral Flow Stripe.
Down to 10 DNA copies/assay.
PCR Mix, rehydration buffer, PCR grade water, positive control, species specific primer, species specific probe, LFA stripe,
LFA running buffer
The Food Control™ kits are designed for the PCR based detection of bacterial DNA. Isolated total DNA from potentially contaminated food serves here as starting material, typically after pre-cultivation of the sample growth medium.
Time to result
PCR reaction tubes, 2.0 ml reaction tubes
Optional: DNA can be extracted from enrichment broth using the DNA extraction kit ExtractNow™ Food Control (Cat. No. 609-1050) or user method.
Required Lab Devices
Racks for reaction tubes, benchtop centrifuge for reaction tubes or PCR plates, vortex, variable microliter pipettes (1 µl – 1000 µl), sterile filter tips, any conventional PCR cycler.
Shelf Life and Storage
Components can be stored at +2 to +8 °C for at least 12 months. After rehydration the reagents must be stored at -18 °C.
No, certification in progress.