Laboratory Diagnostics

---

MB Taq DNA Polymerase

Hot-start MB Taq DNA Polymerase is the optimized mixture of Taq DNA Polymerase and anti- Taq DNA polymerase monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22°C). The inhibition of Taq DNA Polymerase is completely reversed when the temperature is above 70°C. This enzyme is optimal for all Minerva Biolabs products except Onar^®EUB.

Application

Complex genomic or cDNA templates, low copy number targets, large numbers of thermal cycles, multiplex PCR.

Unit Definition

One unit is defined as the amount of the enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble DNA fraction in 30 minutes at 72°C.

Contents and Storage

MB Taq DNA Polymerase, supplied at 4000-5000 units/ml, 4-5 units/µl in 10 mM Tris-HCl (pH 7.0), 50 mM KCl, 0.1 mM EDTA, and 50% (v/v) glycerol. 10x reaction buffer and 100 mM MgCl₂ -solution. Store below -18°C.

EUB Polymerase DNA free

EUB Polymerase is a thermostable DNA polymerase. It has a half-life of 3 hours at 96°C. EUB DNA Polymerase possesses the following activities: 5', 3' DNA polymerase activity and 5', 3' exo-nuclease activity. EUB Polymerase lacks the 3', 5' proofreading activity, which makes it easy to use. EUB Polymerase is purified free of contaminating endo- and exonucleases. This enzyme is optimal for use with Onar^®EUB.

Applications

Complex genomic or cDNA templates.

Unit Definition

One unit is defined as the amount of the enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C.

Contents and Storage

EUB Polymerase, supplied at 100 units/ml, 2 units/µl in 20 mM Tris-HCl (pH 7.4 at 25°C); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5 % Tween 20; 0.5 % Nonidet P 40; 200 µg/ml BSA, and 50 % (v/v) glycerol. Store below -18°C.