Food Control™ qPCR


Food Control™ qPCR is a PCR-kit for fast and reliable detection of foodborne pathogens via real-time PCR.

Detecting bacterial contamination is a key aspect in food microbiology. Consequently, microbiological quality control programs are intensified throughout food chain production in order to minimize the risk of intoxicating consumers. Reliability, speed and robustness of a test system to detect the presence or absence, or even the degree of contamination with pathogens, becomes increasingly important. Food Control™ qPCR is a diagnostic system for easy determination of contamination degree in agricultural or food industry via real-time PCR.


Type of PCR

Taqman® Assay based on FAMTM and HEXTM labeled probes.

Recommended Use / Scope

Can be used in research and industry for detection of pathogenic contamination in food or agricultural products. For research use only! Do not use for clinical diagnostic or testing of patient samples. 

The kit is available for the detection of the following microorganisms via semi-quantitaive real-time or conventional PCR:

Micro-organism Target
Salmonella enterica  
invasion protein A (invA) gen
Yersinia enterocolitica heat stable enterotoxin A gen
Shigella spp. invasion plasmid antigen (ipaH6) gen
Campylobacter spp.

acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine

O-acyltransferase (lpxA) gen

Clostridium perfringens phospholipase C alpha toxin (plc) gen
Shigella dysenteriae,
Escherichia coli (EHEC)
Shiga Toxin 1 (stx1) gen
Shigella dysenteriae,
Escherichia coli (EHEC)
Shiga Toxin 2 (stx2) gen
Escherichia coli O157 wbdR gen
Escherichia coli O104 wckD gen
Listeria spp. invasion associated protein p60 (iap) gen
Listeria monocytogenes listeriolysin O (hly) gen
Salmonella spp.

spacer-region between 16S and 23S RNA genes

Package Sizes

Package size 25 reactions each:

Cat. No.                   Species
11-02-01-025        Salmonella enterica         
11-02-02-025        Yersinia enterocolitica         
11-02-03-025        Shigella spp.             
11-02-04-025        Campylobacter spp.        
11-02-05-025        Clostridium perfringens        
11-02-06-025        Shiga Toxin 1
11-02-07-025        Shiga Toxin 2
11-02-08-025        Escherichia coli O157
11-02-09-025        Escherichia coli O104     
11-02-10-025        Listeria spp.
11-02-11-025        Listeria monocytogenes
11-02-12-025        Salmonella spp.

Result Evaluation

cycler based, real-time PCR

Sensitivity

Down to 10 DNA copies/assay.

Content

qPCR Mix, Species Mix, Rehydration Buffer, PCR Grade Water, Internal Control, Positive Control

Sample Requirements

Isolated total DNA from potentially contaminated food serves here as starting material, typically after pre-cultivation of the sample growth medium.

Required Consumables

PCR reaction tubes, 1.5 ml reaction tubes

Optional: DNA can be extracted from enrichment broth using the DNA extraction kit ExtractNow™ Food Control (Cat. No. 609-1050) or user method.

Required Lab Devices

qPCR cycler with FAM™ and ROX™ filter
variable microliter pipettes
benchtop centrifuge for 1.5 ml reaction tubes

Time to result

150 minutes

Shelf Life and Storage

Components can be stored at +2 to +8 °C for at least 12 months. After rehydration the reagents must be stored at -18 °C.

Compliance

No, certification in progress