Food Control™ LFA


Food Control™ LFA is a PCR-kit for fast and reliable detection of foodborne pathogens via conventional PCR in combination with our reliable Lateral Flow Assay (LFA) evaluation technology.

Detecting bacterial contamination is a key aspect in food microbiology. Consequently, microbiological quality control programs are intensified throughout food chain production in order to minimize the risk of intoxicating consumers. Reliability, speed and robustness of a test system to detect the presence or absence, or even the degree of contamination with pathogens, becomes increasingly important. Food Control™ LFA is a diagnostic system for easy non-quantitative detection of presence or absence of foodborne pathogens in agricultural or food industry via conventional PCR and Lateral Flow Assay (LFA) evaluation technology.


Type of Assay

The assay is based on a one tube amplification with a conventional PCR cycler and a subsequent probe specific hybridization technique. The resulting interpretation is a simple readout of coloured bars on a lateral flow stripe.

Recommended Use / Scope

Can be used in research and industry for detection of pathogenic contamination in food or agricultural products. For research use only! Do not use for clinical diagnostic or testing of patient samples. 

The kit is available for the detection of the following microorganisms via conventional PCR:

Features

Micro-organism Target
Salmonella enterica  
invasion protein A (invA) gen
Yersinia enterocolitica heat stable enterotoxin A gen
Shigella spp. invasion plasmid antigen (ipaH6) gen
Campylobacter spp.

acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine

O-acyltransferase (lpxA) gen

Clostridium perfringens phospholipase C alpha toxin (plc) gen
Shigella dysenteriae,
Escherichia coli (EHEC)
Shiga Toxin 1 (stx1) gen
Shigella dysenteriae,
Escherichia coli (EHEC)
Shiga Toxin 2 (stx2) gen
Escherichia coli O157 wbdR gen
Escherichia coli O104 wckD gen
Listeria spp. invasion associated protein p60 (iap) gen
Listeria monocytogenes listeriolysin O (hly) gen
Salmonella spp.

spacer-region between 16S and 23S RNA genes

Package Sizes

Package size 25 reactions each:

Cat. No.                   Species
11-03-01-025        Salmonella enterica         
11-03-02-025        Yersinia enterocolitica         
11-03-03-025        Shigella spp.             
11-03-04-025        Campylobacter spp.        
11-03-05-025        Clostridium perfringens        
11-03-06-025        Shiga Toxin 1
11-03-07-025        Shiga Toxin 2
11-03-08-025        Escherichia coli O157
11-03-09-025        Escherichia coli O104     
11-03-10-025        Listeria spp.
11-03-11-025        Listeria monocytogenes
11-03-12-025        Salmonella spp.

Result Evaluation

Lateral Flow Stripe.

Sensitivity

Down to 10 DNA copies/assay.

Content

PCR Mix, rehydration buffer, PCR grade water, positive control, species specific primer, species specific probe, LFA stripe,
LFA running buffer

Sample Requirements

The Food Control™ kits are designed for the PCR based detection of bacterial DNA. Isolated total DNA from potentially contaminated food serves here as starting material, typically after pre-cultivation of the sample growth medium.

Time to result

150 minutes

Required Consumables

PCR reaction tubes, 2.0 ml reaction tubes

Optional: DNA can be extracted from enrichment broth using the DNA extraction kit ExtractNow™ Food Control (Cat. No. 609-1050) or user method.

Required Lab Devices

Racks for reaction tubes, benchtop centrifuge for reaction tubes or PCR plates, vortex, variable microliter pipettes (1 µl - 1000 µl), sterile filter tips, any conventional PCR cycler.

Shelf Life and Storage

Components can be stored at +2 to +8 °C for at least 12 months. After rehydration the reagents must be stored at -18 °C.

Compliance

No, certification in progress.