AquaScreen® qPCR Detection kits


The AquaScreen® qPCR Detection kit series is specifically designed for the quantitative detection of common water pathogens such as Legionella, Pseudomonas, and E.coli.The AquaScreen® approach includes sample preparation using the AquaScreen® FastExtract kit and various qPCR detection kits. In contrast to time-consuming cell culture methods, the AquaScreen® approach needs less than six hours including sample preparation and qPCR to reliably detect water pathogens. In addition, our qPCR assay is superior in terms of sensitivity and specificity as the assay only detects the respective species.The AquaScreen® qPCR assay is insensitive to contamination with other bacterial genera. Also, the assay’s robustness is unsurpassed with linear detection up to 3 x 106 particles per sample.


AquaScreen® Legionella pneumophila

Features

Type of PCR
Quantitative real-time PCR (qPCR)

Description
The AquaScreen® Legionella pneumophila qPCR Detection kit is used for DNA samples prepared with the AquaScreen® FastExtract kit for quantification of Legionella pneumophila in waters samples. The used primer set is specific for a segment of the mip region of the Legionella pneumophila genome.

Scope of Application
Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components
Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes
Cat. No. 34-2025    25 reactions
Cat. No. 34-2100   100 reactions
Cat. No. 34-2250   250 reactions

Required Consumables
PCR reaction tubes
Optional: For quantification we recommend our Legionella pneumophila PCR Quantification Standard (Cat. No. 52-0101).

Required Lab Equipment
qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage
The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below -18 °C.

Compliance
AFNOR XP T90-471 and ISO/TS 12869:2012


AquaScreen® Legionella species

Features

Type of PCR
Quantitative real-time PCR (qPCR)

Description
The AquaScreen® Legionella species qPCR Detection Kit is used for DNA samples prepared with the AquaScreen® Fast Extract kit for quantification of Legionella in waters samples. The supplied primer set is specific for a broad range of legionella species, but does not detect other waterborne bacteria as required by ISO/TS 12869:2012.

Scope of Application
Applicable for water testing as described in ISO/TS 12869:2012, in research and industry for QA testing of process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components
Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes
Cat. No. 33-2025    25 reactions
Cat. No. 33-2100   100 reactions
Cat. No. 33-2250   250 reactions

Required Consumables
PCR reaction tubes
Optional: For quantification we recommend our Legionella pneumophila PCR Quantification Standard (Cat. No. 52-0101).

Required Lab Equipment
qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage
The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below -18 °C.

Compliance
AFNOR XP T90-471 and ISO/TS 12869:2012


AquaScreen® Pseudomonas aeruginosa

Features

Type of PCR
Quantitative real-time PCR (qPCR)

Description
The AquaScreen® Pseudomonas aeruginosa qPCR Detection kit is used for DNA samples prepared with the AquaScreen® FastExtract kit for quantification of Pseudomonas aeruginosa in waters samples.

Scope of Application
Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components
Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes

Cat. No. 34-6025    25 reactions
Cat. No. 34-6100   100 reactions
Cat. No. 34-6250   250 reactions

Required Consumables

PCR reaction tubes
Optional: For quantification we recommend our Pseudomonas aeruginosa PCR Quantification Standard (Cat. No. 52-0071).

Required Lab Equipment
qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage
The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below -18 °C.


AquaScreen® Escherichia coli

Features

Type of PCR
Quantitative real-time PCR (qPCR)

Description
The AquaScreen® Escherichia coli qPCR Detection kit is used for DNA samples prepared with the AquaScreen® FastExtract kit for quantification of Escherichia coli in waters samples.

Scope of Application
Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components
Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes

Cat. No. 34-7025    25 reactions
Cat. No. 34-7100   100 reactions
Cat. No. 34-7250   250 reactions

Required Consumables
PCR reaction tubes
Optional: For quantification we recommend our E. coli PCR Quantification Standard (Cat. No. 52-0083).

Required Lab Equipment
qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage
The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below -18 °C.