PCR Quantification Standards


Our PCR Quantification Standards contain genomic DNA extracted from low passage of defined microorganisms. The DNA starting material was analyzed with standardized methods to determine the DNA's integrity and quantity. Only pure and intact DNA showing a > 10 kb band in an agarose gel and an OD260/280 > 1.8 is used. The DNA was partially sequenced to confirm the species' identity.


Scope of Application

PCR Quantification Standards contain a defined number of genome copies of a particular microorganism. Therefore, our standards are perfectly suitable for generating DNA standard curves in qPCR. In addition, standards are required for testing the specificity and sensitivity of PCR assays, particularly, in the context of quality control and in correspondence with EP 2.6.7. compliance

Kit Components

1 vial with 1x108 genomes (freeze-dried.)
3 vials with Tris buffer (10 mM, pH 8.5)

Available Species

  • Cat. No. 52-0116     Acholeplasma laidlawii
  • Cat. No. 52-5571     Bordetella pertussis
  • Cat. No. 52-0083     Escherichia coli
  • Cat. No. 52-0101     Legionella pneumophila
  • Cat. No. 52-0129     Mycoplasma arginini
  • Cat. No. 52-0117     Mycoplasma fermentans
  • Cat. No. 52-0115     Mycoplasma gallisepticum
  • Cat. No. 52-0130     Mycoplasma hyorhinis
  • Cat. No. 52-0112     Mycoplasma orale
  • Cat. No. 52-0119     Mycoplasma pneumoniae
  • Cat. No. 52-0124     Mycoplasma synoviae
  • Cat. No. 52-0164     Spiroplasma citri
  • Cat. No. 52-0071     Pseudomonas aeruginosa

Storage

The kit must be stored at 2-8 °C (Shipped at ambient temperatures).