Contamination Control

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Venor^®GeM- and Venor^®GeM-OneStep-
Mycoplasma Detection Kits

KIT FOR IN VITRO DIAGNOSIS OF MYCOPLASMA

Venor^®GeM and Venor^®GeM-OneStep employ PCR technology for rapid and reliable detection of mycoplasma in various in situ biologicals including cell cultures and virus stocks. For fast, reliable and time-saving routine monitoring!

The Venor^®GeM-OneStep is a complete kit for qualitative direct detection of mycoplasma contaminations in biologicals like cell cultures, sample materials and other culture media. The Kit includes all reagents required for PCR. Primer, nucleotides, polymerase and the internal amplification control are provided ready-to-use  in a lyophylized reaction mix. Just add the included rehydration buffer to the mix, aliquot and add sample or Positive Control DNA and you are ready in one work step.

Ordering

We highly recommend our hot-start MB Taq DNA Polymerase
(Cat. No. 53-0050/ 100/ 200/ 250) for optimal Venor^®GeM performance and most sensitive results. We cannot guarantee excellent results with other polymerases.

Benefits of Venor^®GeM

• Highly sensitive
  Detection of 1,5 copies/µl of mycoplasma DNA that corresponds to 3 mycoplasma per sample volume. LOD_95% = 4,5 copies/µl.
• Specific
  Venor^®GeM is specific for more than 79 mycoplasma species.
• Reliable
  The kit contains internal control DNA and positive control DNA to optimize reproducibility and minimize operator-dependent variability of results.
• Validated
  Venor®GeM was intensively validated according to the guidelines of the European Pharmacopoeia, section 2.6.7.
• Simple
  Templates for PCR analysis are prepared by boiling the supernatant of cell cultures or other sample material.
• Clear
  A clear and easily interpretable result is obtained with one PCR reaction.

Benefits of Venor^®GeM-OneStep

• Convenient
  The Venor^®GeM-OneStep Detection Kit includes the polymerase and the internal amplification control in the lyophylized reaction mix

Test Principle

Minerva Biolabs Mycoplasma Detection Kit Venor^®GeM and Venor^®GeM-OneStep incorporate the highly-sensitive PCR technology as its method for detecting contaminating mycoplasma, which also offers outstanding characteristics over other techniques and products used for mycoplasma safety.

Screen frequently to validate your experimental results.

Screening of cell cultures at regular intervals for mycoplasma contamination is critical for reliable and reproducible results. We recommend to screen cell cultures on a monthly basis as well as before cryopreservation. In addition to our Venor^®GeM and Venor^®GeM-OneStep Mycoplasma Detection Kits, we offer a mycoplasma screening service.

Quality Control Considerations

Mycoplasma are often found as common contaminants in cell cultures, virus stocks, and other cell-derived biologicals. Mycoplasma contamination is a recurring problem that can cause non-reproducible results in research and productivity losses of up to 50 % in industry, which can be avoided by routine diagnostic testing. Nowadays, testing for mycoplasma infection is an essential requirement in reaching quali-ty control standards for all cell culture laboratories. It is necessary to demonstrate quality in laboratories concerned with the production and maintenance of cell lines used in manufacturing various biologicals and in research laboratories to validate their results.

Positive and Internal Controls

Venor^®GeM and Venor^®GeM-OneStep incorporate the highly-sensitive PCR technology as its method for detecting contaminating mycoplasma, which also offers outstanding characteristics over other techniques and products used for mycoplasma safety. The kit includes positive and internal controls. The internal control confirms proper amplification by acting as an alternative template for the PCR primers. Non-infectious Mycoplasma orale DNA is used as a positive control.

Background

Mycoplasma are known to cause a variety of human and animal diseases, and in the laboratory, mycoplasma are often found as common contaminants in cell cultures, virus stocks, and other cell-derived biologicals. Mycoplasma contamination is a recurring problem that can cause non-reproducible results in research and productivity losses of up to 50% in industry, which can be avoided by routine diagnostic testing. Also, testing for mycoplasma infection is now an essential requirement in reaching quality control standards for laboratories concerned with the production and maintenance of cell lines used in manufacturing various biologicals.

Kit Components

• primer sets and nucleotides at optimized concentrations
• 10x reaction buffer optimized for MB Taq Polymerases
• positive control for easy result verification
• internal control to ensure accurate testing
• instruction manual & storage box

We highly recommend our reliable hot-start MB Taq DNA Polymerase for PCR detection of mycoplasma.

Venor^®GeM / Venor^®GeM-OneStep kit components are lyophilized and at +2 bis +8 °C maintainable for at least 12 months. Primer and nucleotides are aliquoted (á 25 tests) to guarantee consistent quality. After rehydratisation the reagents must be stored at -18 °C.

Venor^®GeM / Venor^®GeM-OneStep are intended for research use only. Not recommended for clinical diagnostics or testing of human samples.