Clinical Diagnostics

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>>> MB Taq DNA Polymerase

>>> Swab DNA Extraction Kit

>>> Ordering Information

 

Benefits

• Sensitive
  Detection of less than 15 Treponema pallidum particles per sample.
• Specific
  The Onar^®Tp is highly specific for the polA gene of Treponema pallidum. No cross reactivity is known.
• Reliable
  The kit contains internal control DNA as an optional PCR quality check and positive control DNA to optimize reproducibility and minimize operator-dependent variability of results.
• Convenient
  The convenient master mix format provides all reagents you need for amplification.
• Detection range
  The detection range of the assay is 10 to 10⁶ copies of Treponema pallidum.
• Easy sampling
  The kit provides excellent results with swab samples.

Background

Treponema pallidum is causing Syphilis, also called “Lues”. Syphilis is a curable sexually transmitted disease caused by the Treponema pallidum spirochaete. The organism can also be transmitted to a fetus by transplacental passage during the later stages of pregnancy. Syphilis can be easily treated with antibiotics including penicillin. If not treated, syphilis can cause serious effects such as damage to the heart, aorta, brain, eyes and bones.

 

Kit Components

• Primer, probe and nucleotides at optimized concentrations
• 10x reaction buffer optimized for MB Taq Polymerase
• positive control for easy result verification
• internal control to prevent false negative results by PCR inhibition
• instruction manual
• storage box

 

Test Principle

The Onar^®Tp test system is a test for a qualitative and quantitative diagnostics of in clinical samples. The test is based on the polymerase chain reaction and allows for rapid diagnostics of infections. The supplied primer, probes and the positive control are specific for a segment of the polA gene of the Treponema pallidum genome. When using the Onar^®Tp Detection Kit for real-time PCR the specific target probe is emitting a fluorescent signal at 520 nm. The realtime PCR internal control is detected by another probe emitting fluorescent light at 600 nm. For sampling and sample transport sterile swabs without transport medium should be used (100% Darcon^® or Nylon Swabs). The material can be directly analyzed from the swab. Swabs of ulcer and vaginal smear can be use as test material.