Clinical Diagnostics

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>>> MB Taq DNA Polymerase

>>> Ordering Information

 

 

Instrument Compatibility

 

Instrument	Compatibility
LightCycler® 1.2	+v
LightCycler® 1.5	+
LightCycler® 2.0	+
LightCycler® 480	+
Rotor-GeneTM 3000	+
Rotor-GeneTM 6000	+v
ABI Prism® 7000	o
ABI Prism® 7300	o
ABI Prism® 7500	+
ABI Prism® 7700	o
ABI Prism® 7900	o
iCycler iQ®	o
iQ™5	o
Opticon 2	-
Chromo 4	o
MX3000P®	+
MX3005P®	+
MX4000®	o

 

+ = recommended kit version
– = not compatible
o = untested but presumed to be compatible
v = validated

 

 

Benefits

• Versatility
  Onar^®Pertussis is compatible with the LightCycler^®, Rotor-GeneTM, Opticon^®,
  ABI Prism^®, SmartCycler^®, Mx3000p^® and other devices.
• Sensitive
  Detection of less than 10 pathogen particles per sample volume.
• Specific
  The primers used in the Onar^®Pertussis
  kit are specific for Bordetella pertussis and B. parapertussis.
• Reliable and rapid
  The kit contains a positive control and a homologous internal control for optimal PCR quality check.
• Convenient
  The convenient master mix format only requires a few pipetting steps.

Background

Pertussis, or whooping cough, was a major cause of infant and childhood mortality until the introduction of vaccine programs in the 1940s. In the last two decades pertussis has undergone a resurgence. An increase in incidence has been reported for several countries, whereas an obvious shift in the prevalence of infants and children to adolescents and adults can be observed. Because the symptoms in older and/or previously immunized persons are often atypical, pertussis may be difficult to differentiate from other respiratory tract infections. A reliable and fast in vitro diagnostic system as the Onar^®Pertussis can help preventing further transmission of this highly communicable respiratory disease.

 

Kit Components

• Primer, probe and nucleotides at optimized concentrations
• reaction buffer optimized for MB Taq DNA Polymerase
• positive control for easy result verification
• internal control to ensure accurate testing
• instruction manual
• storage box

Test Principle

The Onar^®Pertussis test system is an in vitro test for the quantitative diagnosis of Bordetella pertussis and/or B. parapertussis in clinical samples. The supplied primer and probe are specific for the dermonecrotic toxin gene (dnt gene) of the genomes of both species. The target probe emits fluorescent light at ~520 nm. To provide assurance that clinical specimens are free of PCR inhibitors, the kit includes an internal amplification control (IAC), that is detected by another probe emitting light at ~610 nm. The IAC is constructed as a homologous control containing primer binding sites identical to the target but a unique internal sequence.