Clinical Diagnostics

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>>> MB Taq DNA Polymerase

>>> Swab DNA Extraction Kit

>>> Ordering Information

 

Benefits

• Sensitive
  Detection of less than 50 MRSA particles per sample.
• Specific
  The Onar^®MRSA Screen is designed to amplify the Methicillin-resistant gene mecA.
• Reliable and rapid
  The kit contains a positive control DNA and a specific internal control probe for optional PCR quality check. Results are obtained within 2-3 hrs.
• Convenient
  The convenient master mix format provides all reagents you need for amplification.
  

Background

MRSA is a specific strain of the Staphylococcus aureus bacterium that has developed antibiotic resistance to all penicillins, including methicillin and narrow–spectrum ß-lactamase-resistant penicillin antibiotics. MRSA can infect other tissues when normal barriers have been breached (e.g. skin). This leads to furuncles, heavy infections and can lead to a sepsis. The Methicillin-resistant Staphylococcus aureus is described as a trigger for typical hospital related infections.

 

Kit Components

• Primers, probes and nucleotides at optimized concentrations
• 10x reaction buffer optimized for MB Taq Polymerase
• positive control for easy result verification
• internal control to prevent false negative results by PCR inhibition
• instruction manual
• storage box

Only purified DNA and MB Taq Polymerase need to be added.

 

Test Principle

The Onar^®MRSA Screen test system is a test for qualitative and quantitative diagnostics of clinical samples. The test can be used with any real time PCR machine suitable with ROX and FAM dyes. The supplied primer, probes and the positive control are specific for a segment of the mecA gene of the Methicillin-resistant Staphylococcus aureus genome. The target probe emits fluorescent light at 520 nm. To rule out the presence of PCR inhibitors in the individual patient specimens, an internal control is included in the kit. The internal control is detected by another probe emitting light at 600 nm.