Clinical Diagnostics

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>>> MB Taq DNA Polymerase

>>> MB DNA Extraction Kit

>>> Ordering Information

 

Benefits

• Sensitive
  Detection of less than 50 MRSA particles per sample.
• Specific
  The Onar^®MRSA is designed to amplify the Methicillin-resistant gene mecA. Staphylococcus aureus is confirmed by detecting the femA gene.
• Reliable and rapid
  The kit contains a positive control DNA and a specific internal control probe for optional PCR quality check. Results are obtained within 2-3 hrs.
• Convenient
  The convenient master mix format only requires a few pipetting steps.

Background

MRSA are specific strains of Staphylococcus aureus which have developed antibiotic resistance to all penicillins, including methicillin and narrow-spectrum ß-lactamase-resistant penicillin antibiotics. MRSA can infect other tissues when normal barriers have been breached (e.g. skin). This leads to furuncles, heavy infections and can lead to a sepsis. The Methicillin-resistant Staphylococcus aureus is described as a trigger for typical hospital related infections.

 

Fig: Sccmec element

(Staphylococcal chromosomal cassette mec)

Methicillin-resistant Staphylococcus aureus are diagnosed by amplification of the mecA gene located within the Sccmec gene locus. The subsequent discrimination of the human pathogenic Staphylococcus aureus and the commensally present Staphylococcus epidermidis will be performed by utilizing the femA gene for amplification.

 

Test Principle

The Onar^®MRSA is a test for semi-quantitative diagnostics of clinical samples. The test can basically be used with all relevant PCR machines. The supplied primer, probes and the positive control are
specific for a segment of the mecA gene of the Methicillin- resistant Staphylococcus aureus genome and the Staphylococcus aureus verification gene femA. The target probe emits fluorescent light at
~ 520 nm and the S. aureus confirmation emits fluorescent light at
~ 610nm. To rule out the presence of PCR inhibitors in the individual patient specimens, an internal control is included in the kit. The internal control is detected by another probe emitting light at ~ 560 nm.

 

Kit Components

• primer, probes and nucleotides at optimized concentrations
• positive control for easy result verification
• internal control to prevent false
  negative results by PCR inhibition
• instruction manual

Only purified DNA and MB Taq Polymerase need to be added.