Clinical Diagnostics

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>>> MB Taq DNA Polymerase

>>> Swab DNA Extraction Kit

 

 

Instrument Compatibility

Instrument	Type 1	Type 2
LightCycler® 1.2	+v	-
LightCycler® 1.5	+	-
LightCycler® 2.0	+	o
LightCycler® 480	+	o
Rotor-GeneTM 3000	+	o
Rotor-GeneTM 6000	+v	o
ABI Prism® 7000	-	+
ABI Prism® 7300	-	+
ABI Prism® 7500	-	+v
ABI Prism® 7700	-	+
ABI Prism® 7900	-	+
iCycler iQ®	o	o
iQ™5	o	o
Opticon 2	-	o
Chromo 4	o	o
MX3000P®	+	o
MX3005P®	+	o
MX4000®	o	o

 

+ = recommended kit version
– = not compatible
o = untested but presumed to be compatible
v = validated

 

 

Benefits

• Sensitive
  Quantitative detection of less than 0.9 IFU/µl. DNA standards are separately available for reliable quantification.
• Specific
  Onar^®Ct is designed to amplify the chromosomal gene momp (major outer-membrane protein). The momp gene is a single copy gene which is suitable for quantification. Furthermore the test detects the new variant Chlamydia trachomatis (nvCT) representing a deletion in the cryptic plasmid, (MØller JK et al., 2008, Alexander S. and Ison C., 2008).
• Reliable and rapid
  The kit contains a positive control DNA and a specific internal control probe for optional PCR quality check. Results are obtained within 2-3 hrs.
• Convenient
  Detailed protocols for different qPCR cyclers and
  sample preparation methods including urine pools allow an instant and simple application of the kit.

Background

Chlamydia trachomatis is an intracellular, gram-negative bacterium. Infection of the lower genital tract is one of the most prevalent sexually transmitted diseases (STD) in the world. Chlamydia trachomatis infection may cause urethritis, cervicitis and pelvic inflammatory disease (PID) in woman. Detecting chlamydial genital infection and preventing transmission and spread of this infection to the upper reproductive tract are a challenge for both clinician and laboratory workers. Nucleotid acid amplification techniques are more used to diagnose chlamydial infections.

 

Test Principle

Onar^®Ct is a test for quantitative diagnostics of clinical samples. The test can basically be used with all relevant PCR machines. The supplied primer, probe and the positive control are specific for a segment of the momp gene of the Chlamydia trachomatis genome. The target probe emits fluorescent light at ~520 nm. To rule out the presence of PCR inhibitors in the individual patient specimens, an internal control is included in the kit. The internal control is detected by another probe emitting light at ~610 nm (Type 1) or ~560 nm (Type 2).

 

Kit Components

• primer, probe and nucleotides at optimized concentrations
• buffer optimized for MB Taq Polymerase
• positive control for easy result verification
• internal control to prevent false negative results by PCR inhibition
• instruction manual
• storage box