The application of real-time PCR techniques in the diagnosis of respiratory infections.

Mycoplasma pneumoniae

Legionella

Current Diagnostic Methods
for Mycoplasma pneumoniae

Current Diagnostic Methods
for Legionella

Course of Infection

New Molecular Target

Principle of PCR
and available tests

Minerva Kits - Step by Step
1 - Sample Preparation

Minerva Kits - Step by Step
2 – DNA Extraction

Minerva Kits - Step by Step
3 – Reaction Setup

Minerva Kits - Step by Step
4 – Cycling

Minerva Kits - Step by Step
5 – Agarose Gel Electrophoresis

Result Evaluation

Technical Features

Looking into the PCR tube

Probe Technology

Real-time PCR Devices

Online-Detection

Probe Technology

Venor^®Mp-QP – Target Detection

Venor^®Mp-QP – Internal Control

Validation-Study I
for Mycoplasma pneumoniae

Validation-Study II
for Mycoplasma pneumoniae

Discrepancies

Features – real-time PCR Kits

Venor^®Mp-QP – Quantification

Interpretation of Quantitative Results

Special Requirements for Sampling and  Sample Transfer

Benefits of PCR in the Diagnosis of Respiratory Infection

General benefits of real-time PCR

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